Phylogenetic and functional characterization of the microbiome of <i>Sargassum</i> seaweed waste

In order to utilize seaweed waste as a feedstock in biorefinery, it is essential characterize the microbial communities and their metabolic processes decomposition. this study, phylogeny functionality of prokaryotic fungal microbiome Sargassum that has inundated Barbados coast were assessed for first time by targeting 16S rRNA gene internal transcribed spacer (ITS) region ribosomal RNA, respectively, using cultivation-independent Illumina next-generation sequencing approach. The resulting 67 032 91 085 high-quality paired-end reads ITS assigned 734 179 amplicon sequence variants (ASVs), respectively. predominant bacterial genera recorded Muricauda, Aminobacterium, Mesorhizobium, Marinobacter, Reichenbachiella, Hyphomonas, Simiduia, Aquamicrobium, Oceanicola Alcanivorax. major Archaea belonged Methanosarcina, Methanogenium Methanosaeta, fungi Sigmoidea, Tylopilus Mucor. functions metagenome, analysed via PICRUSt, revealed pathways related biosynthesis terpenoids polyketides, other secondary metabolites, metabolism cofactors vitamins, xenobiotic biodegradation. Furthermore, prevalence diverse alginate lyase-producing bacteria suggests an ideal niche material isolation potent biocatalysts sustainably depolymerize thereby produce third-generation biofuels bioactive oligosaccharides with novel industrial applications.